Abstract
The purpose of this study was to understand the therapeutic effect and antibiotic pus transfer under the set conditions of an experimental buccal abscess model.
[Methods]
Male Wistar strain rats weighing about 300g were used. For the inoculation with bacteria strains of Streptococcus pyogenes S-8 were employed. S. pyogenes was cultured in trypto-soy broth for 18 h at 37°C and prepared 107 at CFU/ml. A bacterial inoculation fluid containing 0.1 mg/ml of Epinephrine (Epi) and 1% Carboxymethyl cellulose (CMC) of which 0.1 to 1 ml was intracutaneously injected into the buccal area, was prepared. Comparative observations with the control group which did not receive Epi or CMC were made.
After formation of the abscess, the antibiotic Cefoperazone (CPZ) at 40 mg/kg were intraperitoneally injected. By using the bioassay method, the serum pus concentrations at 15 and 30 min, and at 1, 2 and 4 h intervals after administration were determined. The determined values underwent pharmacokinetic analysis based on a one or two compartment open model and various coefficients were found. Also, at 2, 4, 24 and 48 h intervals, the number of living bacteria was determined.
An in vitro model system was set up to simulate the pus concentration, and the number of living bacteria were determined up to the fourth hour.
[Results]
The abscess formation reached a peak 24 h after inoculation of the bacteria. In the 0.1 mg/ml Epi and the 1% CMC groups, 0.5 ml was the appropriate amount of inoculated bacteria. By the 24th h following inoculation of the bacteria, CPZ was intraperitoneally administered and 15 min later it reached the peak value of 41.20μg/ml. Thereafter, it decreased rapidly until by the time of the fourth hour it was below the level of measurability. The peak value for pus was seen after 30 min (19μg/g) . After 15 min, this value was 18μg/g, after 1 h 9.81 μg/g, after 2 h 5.70μg/g and after 4 h 3.19μg/g. Between the blood serum and the pus a value based on the actual measurement having a correlation of Y=4.17+0.36 X (r=0.98) was found.
In order to study the therapeutic effect of the drug, the number of live bacteria within the pus was determined. It matched well with the bactericidal effect which was studied for comparison with a simulated in vitro model of the pus concentrations.
