主催: The Japanese Society of Plant Physiologists
p. 345
The E.coli gene katE, driven by the promoter of Rubisco small subunit gene of tomato, rbcS3C, was introduced into a tomato (Lycopersicon esculentum Mill.) by Agrobacterium tumefaciens-mediated transformation. Catalase activity in transgenic plant was approx. 3-fold higher than that in wild-type plant. Leaf discs from transgenic plants remained green at 24h after treatment with 1 μM paraquat under moderate light intensity, while leaf discs from wild-type plants showed severe bleaching after the same treatment. In addition, ion leakage from transgenic leaf discs was significantly less than that from wild-type leaf discs at 24h after treatment with 1 μM paraquat and 10 mM H2O2, respectively, under moderate light intensity. The protect efficiency of the E.coli catalase in the transgenic plant was checked against different oxidative stresses. The transgenic plants showed relatively higher tolerance for paraquat, exogenous H2O2 and cold stress than the wild-type plants but not for salt stress
