抄録
To investigate the whole genetic system of legumes, we initiated
large-scale sequencing of the genome of a model legume, Lotus
japonicus. Using the information on ESTs and cDNA markers of
legume plants, genomic clones corresponding to the multiple seed
points of the genome have been chosen as initial targets. As the
accumulation of the seed sequences has progressed, clone selection by
walking has also initiated. Currently, a total of 1,550 clones have
been selected. One hundred and twenty seven of them are in the library
phase, 952 are in the finishing phase and 412 have been annotated. A
total of 3,975 protein coding genes were identified in 42.2
Mbp. Mapping of the seed clones has also been performed by generating
PCR-based markers from sequence information, and 1,140 clones have
been located on the linkage map. The sequence data, gene information
and mapping information are available through the WWW at
http://www.kazusa.or.jp/lotus/.
