日本植物生理学会年会およびシンポジウム 講演要旨集
第45回日本植物生理学会年会講演要旨集
会議情報

タバコ由来の膜結合型蛋白質プロリン残基水酸化酵素
Koji YuasaHiroo Fukuda*Ken Matsuoka
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会議録・要旨集 フリー

p. 599

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抄録
We cloned a novel prolyl 4-hydroxylase (PH; EC 1.14.11.2) homologue cDNA from tobacco BY-2 cells based on expression sequence tag information. Like other PHs, this tobacco PH polypeptide has two conserved histidine residues. It consisted of 286 amino acids with a calculated molecular mass of 32 kDa. This protein and homologues in Arabidopsis and rice have possible transmembrane regions at their N-terminus. This PH homologue was expressed in BY-2 cells as a His-tagged protein, and the expressed protein showed PH activity. Incubation of membranes with high salt, urea and detergents indicated that this protein was an integral membrane protein with a type II configuration. The tobacco PH formed several large protein complexes devoid of protein disulphide isomerase in the membrane.
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© 2004 日本植物生理学会
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