抄録
Rice PLASTOCHRON1 gene encodes a cytochrome P450 protein, the CYP78A11. Loss-of-function mutations of the PLA1 affect the plant height, lamina joint bending, leaf initiation rate and the duration of vegetative phase. Transgenic plants over-expressing PLA1 exhibit abnormal leaf development resulting in seedling lethal. Phenotypic analysis of pla1 mutant suggested that PLA1 might regulate rice development via the regulation of plant hormone(s). Plant P450s are known to be involved in important biochemical pathways such as biosynthesis of alkaloids, terpenoids, lipids, glucosinolates, and plant growth regulators such as gibberellins, auxins, and brassinosteroids. To investigate the biochemical changes associated with PLA1 function, we performed metabolome analysis (Phenomenome Discoveries Inc.) using PLA1 over-expressing plants. It was revealed that over-expression of PLA1 could cause global change in the rice metabolism, including tryptophan metabolism and terpenoid biosynthesis that are related to auxins and gibberellins production, respectively. Further analysis is now under way.
