Abstract
Plant metabolomic approach, in the post-genome era, has attracted considerable interests in light of the fundamental importance not only for understanding plant metabolisms but also for unlimited potentials of plant metabolites in both crop quality improvement and industrial applications. In this study, we investigated non-biased metabolite analyses with the aid of FT-ICRMS (Fourier Transform-Ion cyclotron Resonance-Mass Spectrometry) together with the combination of GC/MS and LC/MS. In the FT-ICRMS, we were able to observe more than 1000 m/z values (mass peaks) from each analysis of 100% methanol extracts, and the results were subjected to PCA. Metabolic profiles were obtained from Arabidopsis plants with a T-DNA insertion into a cytochrome P450 gene (CYP51) and from Arabidopsis cultured cells treated with P450 inhibitors as well. The PCA data are discussed with the changes in the target gene expression and related metabolic pathway activities.
