抄録
Within the genus Populus, Populus euphratica is highly salt tolerant. Under salt stress leaves develop succulence and the wood acquires anatomical changes. Furthermore, H2O2 is produced in roots indicating that salt stress results in oxidative stress. Based on this observation, we hypothesize that H2O2 is a second messenger in differentiation processes under oxidative stress. To test this assumption thematic arrays containing 449 ESTs from Populus euphratica were hybridized to RNA from roots of different developmental stages under salt stress. We focused on those genes which are related to H2O2 production and oxidative stress (e. g. oxidases, peroxidases, catalases) and those related to lignification (e. g. cinnamyl alcohol dehydrogenase, 4-coumarate CoA ligase) to get first hints for links between oxidant production and anatomical changes. Furthermore, to get further insights into the regulation and to identify new candidate genes microarrays studies with arrays haboring a unigene set (7745 ESTs) are currently underway.
