Abstract
Production of reactive oxygens (ROS) is a commonly observed defense response by higher plant infected with pathogens. ROS plays a variety of roles such as cross-linker of cell wall, signal molecules for the following defense reactions, and fungicidal activities in vitro. However, little is known on the defense mechanisms in pathogens against ROS produced by the host. We detected catalase-like activity in the culture filtrate of rice blast fungus. The activity disappeared by heating at 100 and was detected in the absence of electron acceptor, indicating that it is catalase-like activity. Western blot analysis of total protein in the culture filtrate with anti bovine catalase did not detect positive signals. We will report some characterization and trial to purify the enzyme.
