Abstract
Lipid rafts are relatively resistant to solubilization at low temperature in nonionic detergent. These domains are known to be enriched in sterols and sphingolipids and postulated to be involved in transmembrane signaling. The aim of this study is to analyze the components of lipid raft domains associated with defense responses of rice.
First, we purified lipid raft proteins from rice cultured cells by using Triton-X 100 and sucrose density-gradient ultracentrifugation and we are now identifying the proteins in the raft fraction by mass spectrometry. Also, as our previous studies indicated that OsRac1, a small GTP-binding protein, functions as a key regulator of defense signaling in rice, we analyzed the raft fractions isolated from constitutive active OsRac1 (CA-Rac) and dominant negative-OsRac1 (DN-Rac) expressing rice cells. We investigated whether the raft components among Wild type, CA-Rac and DN-Rac cells were different.
