Abstract
To improve utility of tobacco BY-2 cells as an experimental tool we had been collecting ESTs and had analyzed expression profiles using a custom-made microarray made of the EST clones. However, there are limits in the usefulness of EST clones and sequences. To overcome this problem we are analyzing complete length cDNAs of BY-2. Library was made by V-cappping method using total RNAs prepared from log, stationary and jasmonate-treated cells. After sequencing of several thousand clones from the 5' terminus, we obtained approx. 2200 unique complete-length cDNAs. Using the sequence information of these clones, of previously analyzed EST and of other genes of tobacco found in public database, we are now constructing high-density oligo-nucreotide array using an on-chip synthesis method. In this presentation the result of the sequencing of full-length cDNA clones and the progress of the construction of oligo-array will be presented.
