Abstract
Plant hormones have been known as signal compounds which regulate various responses at a low concentration in plants. To reveal the system, endogenous level of them had been quantified mainly by using GC-ESI-MS. Recently, microanalysis by using LC-ESI-MS/MS have been carried out frequently. In this method, samples do not require complicated purification and derivatization that GC-EI-MS requires. However, because ionization efficiency in LC-ESI-MS/MS method is susceptible to impurities, it requires suitable purification.
Our objective is that establishment of method for comprehensive analysis of all plant hormones. Here we report the method for analysis of gibberellin, abscisic acid, indole-3-acetic acid, jasmonic acid and salycilic acid. In our method for each hormone, required amount of plant material is 1/1000 to 1/10 to that of GC-EI-MS analysis and it requires no HPLC purification.
We will also report the analysis of other hormones and the comprehensive analysis from 100 mg of plant material.
