Host: The Japanese Society of Plant Physiologists
Pages 0132
Plants have adaptive abilities for changing environment by metabolic balance to keep internal homeostasis. To follow this system, we are developing simple methodologies to detect time-dependent changes of metabolites using Arabidopsis T87 cells. Firstly, we performed 1H-NMR metabolic profiling of cellular metabolites upon treatments with biosynthetic intermediates. Secondly, T87 cells were uniformly labeled with [13C6] glucose and the 13C labeled low molecular metabolites were identified with a combination of multi-dimensional NMR methods such as 1H-13C-HSQC, HCCH-TOCSY, and HCACO. Thirdly, we are trying to extract the basic information of metabolic flux with the labeled metabolites by 13C -13C coupling constants in 1H-13C -HSQC. Additionally, we have examined how the 13C metabolic flux could be different in insoluble macromolecule and soluble low molecular metabolites since energy accumulation and consumption in plant cells are essential issues for food and energy sources. We will discuss future perspectives of the 13C flow monitoring method.
