Abstract
The phytohormone auxin is transported by polar auxin transport system from cell to cell, leading to the asymmetric auxin distribution. Polar auxin transport is dependent on the activity of auxin efflux carriers, PIN-FORMED (PIN) proteins, localized in the plasma membrane with polarity. Recently, the Ser/Thr kinase PINOID (PID) and NONPHOTOTROPIC HYPOCOTYL 3 (NPH3)-like protein MACCHI-BOU 4/ENHANCER OF PINOID (MAB4/ENP) have been reported to regulate polar auxin transport through the control of subcellular localization of PIN proteins. Although the MAB4/ENP protein has NPH3 and BTB/POZ domains, the function remains to be clarified. To isolate genes encoding proteins that interact with MAB4/ENP, we carried out a yeast two-hybrid screen. As a result, we identified several candidate genes. This time, we will present the results of our research on these genes. In addition, analysis results on MAB4 family genes, redundant with MAB4/ENP, will be also presented.
