Abstract
Lateral root (LR) formation is important for the construction of root system in higher plants. It is known that auxin promotes LR formation but the regulatory mechanism is not fully understood. We have studied the molecular cascade regulating auxin-mediated LR formation using Arabidopsis. From the molecular genetic analysis of several mutants defective in LR formation, we have shown that Auxin Response Factor 7 (ARF7) and ARF19 regulate LR formation via auxin-inducible transcription of LATERAL ORGAN BOUNDARIES domain 16 (LBD16) and LBD29 genes, encoding nuclear proteins (Okushima et al., 2007). Because these LBDs are thought to be transcriptional regulators, we tried to identify the downstream genes of LBD16 using DNA microarray. In this poster, we will present the results of expression analysis and functional analysis of several candidate genes.