Abstract
To understand the molecular mechanism underlying the tolerance to acidic stress of wild plants growing in the acid sulfate soils in Southeast Asia, comprehensive analysis of acidic stress-responsive genes of Panicum repens (Poaceae) and Acacia mangium (Fabaceae) were carried out by means of differential display approach. Roots of P. repens and callus of A. mangium were cultured using the media adjusted to pH 6.0 and pH 3.0 with sulfuric acid. Total RNA was extracted one and 24 hours after acidic treatment and RT-PCR was perfromed with random primers. As the results of the PCR, 127 up- and 61 down-, and 80 up- and 32 down- regulated fragments of acidic stress-responsive genes were detected for P. repens and A. mangium, respectively, and were cloned and sequenced. Many genes involved in the energy metabolism, amino acid synthesis, transport, signaling, and transcription were commonly detected from the distantly related species.
