Abstract
We designed a novel tiling array, AtMap1, for genomic deletion mapping. The AtMap1 is a 60-mer oligonucleotide microarray consisting of 42,497 data probes designed from the genomic sequence of Arabidopsis thaliana Col-0. The performance of the AtMap1 array was assessed using the mag2-2 mutant, which is a well-studied deletion mutant. Eight of the probes showed 3-fold lower signals in mag2-2 than Col-0. Seven of these probes were sequentially located on chromosome 3. This deletion included previously reported deleted region. The other probe was located near the end of chromosome 4. A deletion around the probe was confirmed by PCR. This deletion was newly identified in this study. Thus, we concluded that the AtMap1 array was sufficiently sensitive to identify a deletion without a priori knowledge. Mutation mapping is time-consuming, laborious and costly. The AtMap1 array removes the difficulties.
