Abstract
Compared with genomes, a large amount of EST data is available for eukaryotes including plants. We have provided web tools to annotate genomes and reconstruct pathways for eukaryotes using EST data. By applying the tools to ESTs for over 40 plant species, we created pathway data and they are available as the KEGG EGENES database along with the assembled gene sequences. EGENES are constructed in two steps. First by extracting EST data of each plant species from dbEST, a virtual gene set is created after clustering and assembling the ESTs. Second, automatic annotation of the genes is performed for further pathway reconstruction. Both steps are available as web tools called EGassembler and KAAS (KEGG Automatic Annotation Server), respectively, and anyone can access to them to perform pathway reconstruction from EST sequences. I would like to introduce the tools and an application of the EGENES.
