Abstract
Photosynthetic organisms have developed additional antennae that function in the collection of light energy to support photochemistry in reaction centers. In green plants LHCI and LHCII serve for photosystem I and II, respectively. These antenna systems respond to changeable environmental conditions immediately and manifest effects for a long period of time, adjusting light absorption and also contributing to the removal of excess light energy. In this study we focus on the regulation of the antenna system and aim to identify genes involved on such regulation.
Arabidopsis thaliana plants grown at 70 μE or 230 μE for 10 days were used for 2-D analysis of chlorophyll fluorescence with FluorCam. So far, we have screened about 1,000 T-DNA/Ds inserted lines. At the 68.3% significance level on two photosynthetic parameters, ΦII and NPQ, 620 candidates were identified, including 90 knockout lines of genes encoding unknown proteins. Taking into consideration the genomic information of the plant samples used, about 400 lines have been extracted and while further analysis is currently being undertaken. The detail will be presented on our next meeting this coming March 2010.
