Abstract
Linkage maps of cultivated tomato were constructed to promote post-genome sequencing studies in tomato. We discovered 5,607 candidate SNPs by comparison of 49,972 contigs that derived from 229,086 ESTs registered in public databases. Out of the 5,607 candidate SNP markers, 1,536 were tested for polymorphism using Illumina GoldenGate system for two mapping populations, namely AMF2, consisting of "Ailsa Craig", "Micro-Tom", and its F2 progeny, and MMF2, consisting of "M82", "Micro-Tom", and its F2 progeny. As the results, a total of 646 and 639 SNP markers showed polymorphism in the AMF2 and the MMF2 populations, respectively, and were subjected to linkage analysis. A linkage map of the AMF2 consisted of 991 loci, including 646 SNPs and 345 SSRs, which were previously developed from EST and BAC-end sequences, and covered 1,468 cM. In the MMF2 population, a total 637 SNP loci were located onto 12 linkage groups representing 1,126 cM. The results in this study will contribute the advances in physiology, molecular genetics, and genomics in tomato.
