Abstract
In order to investigate the whole genetic system of legume species, we have been analyzing the genome of a model legume, Lotus japonicus.
In 2008, we have released the 315 Mb sequences determined by combination of two independent approaches; clone-by-clone sequencing of large insert clones and random sequencing of selected genomic regions. We continued our efforts on sequencing of genome clones, and the sequence information on 460 clones has been accumulated. Thus, we have updated the sequence information by adding these clone sequences. As a result, the total length of the pseudomolecule, which means genome regions covered by genome clone sequences, was increased from 167 Mbp to 201 Mbp, and the total size of finished (phase 3) sequences was increased from 97 Mbp to 139 Mbp. Using the updated genome sequence and predicted gene information, we have carried out comparative genome analysis against Medicago truncatula and soybean. As a result, high level of syntenic relationship was identified within these legume species. The detailed information on the updated sequences and annotation will be available through our web database, "miyakogusa.jp" (http://kazusa.or.jp/lotus/).
