Pseudomonas syringaeの遺伝子操作と農業利用(組換え微生物の農業利用-野外での利用を中心にして-)

抄録

Recent developments of molecular genetics relating to pathogenicity factors in Pseudomonas syringae pathovars, such as pv. atropurpurea, pv. savastanoi, pv. phaseolicola, pv. syringae and pv. glycinea were reviewed. Coronatine production of pv. atropurpurea is controlled by plasmid genes of this bacterium. The plasmid designated as pCOR1 was excluded at a high frequency in plants or by co-cultivation with plant cells, along with the loss of pathogenicity. Moreover, fertility profiles of P. syringae pathovars including pv. tabaci, pv. mori, pv. tomato, pv. lachrymans, etc. were described. The plasmid pBPW1 of pv. tabaci was highly conjugative and affected the antigen alteration of a strain of pv. atropurpurea. The plasmid was also able to mobilize the pCOR1 plasmid into avirulent strains of pv. atropurpurea in mating experiments in planta, indicating that virulence genes were transferred between isolates of plant pathogenic pseudomonads.