In the multileveled bio-engineering including amino-acids, proteins, organelle, cells, tissues, and organs, control of hetero-biointerfaces is a key technology. In particular, recent development of nanotechnology has made the nanoscaled control possible. In this article, we selected current topics in the hetero-biointerfaces. In the layer-by-layer stacking technique of cells, artificial tissues similar to a blood vessel has been realized. Phospholipid polymers similar to cell membrane were used to control the biomaterial surfaces. Properties of their interfaces with proteins are analyzed focusing on functions of the interface water layers. Selective adsorption of protein molecules on a solid surface is also demonstrated. In the biointerface engineering, nanoscale control play crucial roles.
