Phylogenic Analysis and Serotyping of Rice yellow mottle virus Strains in Uganda

抄録

Rice yellow mottle virus (RYMV) is a major rice (Oryza spp.) virus found only in Africa. In the present study, we collected samples of rice mainly from Eastern Uganda showing RYMV symptoms. The presence of RYMV was confirmed using ELISA and PCR. Some RYMV isolates in Uganda were sequenced and showed high homology levels with the Madagascar RYMV strain. They were grouped into S4ug strain in the East African lineage, showing high homology levels with isolates from neighboring countries. Serotyping based on the amino acid sequences of a RYMV coat protein gene fragment enabled to classify the Uganda isolates into Ser 4, in accordance with a previous serotyping report of other Ugandan isolates, using monoclonal antibodies. The present study revealed the widespread occurrence of RYMV in Uganda with a low diversity, irrespective of the origin of location and the rice varieties, as well as the first serotyping of Ugandan RYMV based on the amino acid sequences but not on monoclonal antibodies.