2008 Volume 58 Issue 2 Pages 117-124
Live-cell imaging of cells infected with recombinant viruses expressing fluorescently tagged structural viral proteins enables to visualize virion maturation pathways at a virion particle level in the same cells as infection progresses. This technology have gradually unveiled previously unreported aspects of the pathways. This article focuses on live-cell imaging technology of herpes simplex virus 1 (HSV-1) and reviews the up-to-date topics of HSV-1 maturation pathway including our recent progress.