VIRUS
Online ISSN : 1884-3425
ISSN-L : 1884-3425
インフルエンザウイルスの精製に関する研究
I. 各種吸着剤並に沈澱剤による精製 (1)
赤司 暸雄
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ジャーナル フリー

1954 年 4 巻 2 号 p. 122-127

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抄録
The partial purification of influenza virus was recognized to be given to a certain degree, by absorption and elution using such absorbents as ion-exchange resins, clay, kaoline, diatom earth, or chicken red cells. Furthermore, purification was obtained in considerably high degree, by combining precipitation method to the materials, preliminarily purified by these absorbents, or by combining methanol precipitation method as well as a few absorbents.
In a experiment in which methanol precipitation method was combined with the purification procedure by chicken red cells, applied to the supernatant fluid obtained by the absorption to and elution from ionexchange resin, the following result was obtained: In the final purified material, the concentration of the virus, designated by the hemagglutination titer, increased by 4 times and the nitogen content decreased to 99.4% compared with those of the original material.
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