1955 Volume 5 Issue 2 Pages 83-89
One of the pair of two mice, which had been brought in parabiosis, was immunized against the Japanese B encephalitis with the repeated intracerebral inoculation of the inactivated virus. The titer of the serum neutralizing antibody rises significantly by this procedure not only in this immunized donor mouse but also concomitantly in the parabiont by means of the blood circulation which had been made common to the both of the parabiosis. In this case the titer of the neutralizing antibody contained in the brain tissue, from which the circulating blood had beforehand been thoroughly washed away by the perfusion of the brain vessels with saline solution, also turn out higher in the brain tissue of the donor mouse parallel with that of the serum antibody, but not in that of the parabiont. In the single mice, which had beforehand been immunized with the similar procedures, the antibody can be proved in significantly high titer in both the serum and the perfused brain tissue. After the parabiosis of these mice with the normal, the serum antibody titer rises also in the latter up to at least the same level with the former by means of the blood circulation common to the both, but that of the brain tissue remains almost unchanged. Even when the parabiont mice received repeated intracerebral inoculations of the normal mouse brain (N. M. B.), concomitantly with the inoculations of the inactivated virus to the donor mice, the antibody appears only in the brain tissues of the donors and not in that of the parabionts, although the titer of the antibody rises to almost the same level in the both.
Standing upon the data described above, it might be possible to conclude that by the intracerebral inoculation of the inactivated Japanese B encephalitis virus, the intracerebral concentration of the neutralizing antibody against it takes place in loco, and still more, that the simple mechanical injuries of the brain such as the repeated intracerebral inoculation of the N. M. B. does not cause the permeation into and the adhesion to the cerebral tissues of the serum antibody, which might be ascribable to the rise of permeability of the so-called blood-brain-barrier caused by the injuries. Namely it might be possible to conclude that in the intracerebral concentration of the neutralizing antibody the direct intracerebral inoculation of the antigen plays a most important role as one of the substantial cause of it.
