STUDIES ON THE PURIFICATION OF INFLUENZA VIRUS (II)
PURIFICATION OF INFLUENZA VIRUS BY USE OF PRECIPITANTS
1955 Volume 5 Issue 2 Pages 89-100
Details
1955 Volume 5 Issue 2 Pages 89-100
The purification of influenza virus by the various absorbents and precipitants was reported in the previous paper, and it was ascertained that influenza virus could be partially purified in some degree dy ZnCl2, KAl (SO4)2, protaminsulfate and isoelectric precipitation.
Furthermore, it was recognized that influenza virus partially purified in some degree by application of various absorbents and precipitants could not be sufficiently precipitated under the conditions suitable to precipitate that of crud infected allantoic fluid, but sufficiently precipitated by changing the conditions.
In the experiments shown here, however, it was confirmed that influenza virus partially purified from the infected allantoic fluid, diluted to 10% in buffer solution, was almost all precipitated when treated by methanol in the concentration of 30 per cent for 10 hours or by that in the concentration of 33 per cent for 5 hours. In both cases, pH was 7.2.
The harvest of influenza virus from the sediment was not so good when eluted by low concentration of phosphate buffer, but it was confirmed that virus was completely harvested when eluted by 0.2M-0.3M phosphate buffer.
Thus, pretty good results were obtained in the purification experiments by application of methanol precipitation method to the partially purified virus obta ned by precipitation with ZnCl2, KAl (SO4)2, protaminsulfate, and isoelectric method.
