Abstract
Salmonella anatum of group E1 was at first lysogenized with phage ε34 under particular conditions (Uetake and Hagiwara, Nature 186, 261, 1960a; Virus, 1960b in press). The resulting ε34-lysogenic cells (=A(ε34)) with antigens 3, 10 were then lysogenized with one of phages, ε15a, ε15b and εy, all of which are mutants of phage ε15 with abnormal conversion properties (Uetake and Uchida, Virology 9, 495, 1959). The resulting double-lysogenic derivatives A (ε34, ε15a), A (ε34, ε15b) and A (ε34, εy) are resistant to phages C341, ε15vir, ε34vir and ε34vir h, although cells A (ε34, ε15b) still adsorb phage ε34vir and cells A(ε34, εy) adsorb phages C341 and ε15vir.
Agglutination and absorption tests showed that somatic antigens of A (ε34, ε15a), A (ε34, ε15b), and A(ε34, εy) were (3), (10), (15), (34), 3, (10), (15). (34), and (3), (10), (15), (34) respectively, different from each other. These indicate that converting properties of ε15a, ε15b, and εy are different from each other, consistent with the previous findings (Uetake and Uchida, 1959) except the finding that phage εy was revealed to retain some gene (s) responsible for the synthesis of antigen 15. Since the synthesis of antigen 34 is always accompanied by the synthesis of antigen 15, they also indicate that the machinery for the synthesis of antigen 15 is a necessary prerequisite for the formation of antigen 34, as pointed out in previous papers (Uetake and Hagiwara, 1960a, 1960b).
