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Volume 10, Issue 5
Displaying 1-6 of 6 articles from this issue
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HISAO UETAKE, SNIN HAGIHARA1960 Volume 10 Issue 5 Pages 285-289
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSSalmonella anatum of group E1 was at first lysogenized with phage ε34 under particular conditions (Uetake and Hagiwara, Nature 186, 261, 1960a; Virus, 1960b in press). The resulting ε34-lysogenic cells (=A(ε34)) with antigens 3, 10 were then lysogenized with one of phages, ε15a, ε15b and εy, all of which are mutants of phage ε15 with abnormal conversion properties (Uetake and Uchida, Virology 9, 495, 1959). The resulting double-lysogenic derivatives A (ε34, ε15a), A (ε34, ε15b) and A (ε34, εy) are resistant to phages C341, ε15vir, ε34vir and ε34vir h, although cells A (ε34, ε15b) still adsorb phage ε34vir and cells A(ε34, εy) adsorb phages C341 and ε15vir.
Agglutination and absorption tests showed that somatic antigens of A (ε34, ε15a), A (ε34, ε15b), and A(ε34, εy) were (3), (10), (15), (34), 3, (10), (15). (34), and (3), (10), (15), (34) respectively, different from each other. These indicate that converting properties of ε15a, ε15b, and εy are different from each other, consistent with the previous findings (Uetake and Uchida, 1959) except the finding that phage εy was revealed to retain some gene (s) responsible for the synthesis of antigen 15. Since the synthesis of antigen 34 is always accompanied by the synthesis of antigen 15, they also indicate that the machinery for the synthesis of antigen 15 is a necessary prerequisite for the formation of antigen 34, as pointed out in previous papers (Uetake and Hagiwara, 1960a, 1960b).View full abstractDownload PDF (778K) -
MINORU SHIBUKI1960 Volume 10 Issue 5 Pages 289-304
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSThirteen strains of distemper virus were isolated in ferrets from the spleens of 31 dogs clinically diagnosed as distemper. Serial passage in developing chicken eggs was attempted with all the strains and 3 were successfully adapted to this host. Propagation in chicken embryos of the egg-adapted virus was studied under various experimental conditions with one of the isolated strains and inoculation into the yolk sac of embryonating eggs incubated for only a few days was found to give the greatest yield of the virus. Inoculation of active virus of the egg-adapted strain produced immunity in ferrets and dogs as revealed by protection test and serum neutralization test. The relationship of the amount of inoculated virus and the extent of acquired immunity was studied.View full abstractDownload PDF (2674K) -
HIROSHI OGIWARA1960 Volume 10 Issue 5 Pages 305-315
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSThe serological response of guinea pigs to mumps virus was studied following inoculation of active virus of the Enders strain and the electrophoretic study was performed with the antiserums obtained, with the intention of obtaining antiserums to S antigen devoid of V antibodies and those to V antigen devoid of S antibodies.
1. Following intracerebral, intranasal, or intraperitoneal inoculation of active virus guinea pigs developed antibodies to V antigen as well as to S antigen.
2. Guinea pigs which developed V and S antibodies following intranasal inoculation of active virus were injected with S antigen prepared from infected chorioallantoic membrane by centrifugation or V antigen prepared by the ether treatment of virus particles to remove the internal S antigen 53 days following the intranasal inoculation, when anti-V and anti-S levels became very low. The booster injections elicited rapid production of a large amount of antibodies to the homologous booster antigen, whereas a little response to the heterologous antigen was noted. By this method high-titered anti-S serums, almost devoid of anti-V, or vice versa, were obtained.
3. The starch zone electrophoresis revealed the presence of V and S antibodies in the gammaglobulin fraction. V and S antibodies showed a slight difference in electrophoretic mobility, which made the separation of V and S antibodies possible.View full abstractDownload PDF (1482K) -
TOMIAKI MORIMOTO1960 Volume 10 Issue 5 Pages 316-329
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSThe virus of Russian spring summer encephalitis (RSSE) multiplied without any cytopathogenic effect (CPE) in cell culture of bovine embryonic kidney. Newcastle disease (ND) virus proliferated with CPE in this cell culture, but was not able to proliferate and produce CPE in cell culture previously infected with RSSE virus. With small doses of RSSE virus it took about 4 days for the majority of cells in culture to acquire the ability to interfere with ND virus. Interfering activity of RSSE virus could be detected earlier with a smaller inoculum of ND virus than with a larger one, but the inoculum size of ND virus did not affected the result when ND virus was given at a certain interval following RSSE virus inoculation or later.
Interference between these two viruses were observed in renal cell cultures of the swine, cat, rabbit, and guinea pig, and not in cell cultures of hamster and chicken kidneys or chick embryo tissues. RSSE virus interfered with fowl plague virus and almost or entirely not with viruses of vaccinia, herpes, equine abortion, Venesuelan equine encephalitis, and bovine infectious rhinotracheitis, and HVJ (hemagglutinating virus of Japan).
Japanese encephalitis virus and Negishi virus also interfered with ND virus in bovine embryonic renal cell culture.
Interference of RSSE virus with ND virus was specifically neutralized by immune serum to RSSE virus.
Based on these findings a procedure was established for detecting and measuring RSSE virus and its neutralizing antibody.View full abstractDownload PDF (3280K) -
YUKIMASA HAYASHI, TOKUZO HIRAI1960 Volume 10 Issue 5 Pages 330-337
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSThe present paper concerns the alteration of nitrogen metabolism of TMV-infected tobacco leaf discs induced by some antiviral substances using detached leaf tissue-culture procedure. The amount of soluble protein nitrogen in infected leaves decreased in the early stage of infection, and later increased with a stage of corresponding decrease of the amount of non-protein nitrogen and of increase of virus titer. 2-Thiouracil and mitomycin C-treatments altered this trend to the levels of uninfected tissues and a significant accumulation of insoluble protein component was observed. The inhibitory action of naramycin was different from this, and an increase in the amount of non-protein nitrogen and a decrease in that of insoluble protein nitrogen were characteristic.View full abstractDownload PDF (1211K) -
1960 Volume 10 Issue 5 Pages 341-358
Published: 1960
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (3334K)
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