Abstract
The serological response of guinea pigs to mumps virus was studied following inoculation of active virus of the Enders strain and the electrophoretic study was performed with the antiserums obtained, with the intention of obtaining antiserums to S antigen devoid of V antibodies and those to V antigen devoid of S antibodies.
1. Following intracerebral, intranasal, or intraperitoneal inoculation of active virus guinea pigs developed antibodies to V antigen as well as to S antigen.
2. Guinea pigs which developed V and S antibodies following intranasal inoculation of active virus were injected with S antigen prepared from infected chorioallantoic membrane by centrifugation or V antigen prepared by the ether treatment of virus particles to remove the internal S antigen 53 days following the intranasal inoculation, when anti-V and anti-S levels became very low. The booster injections elicited rapid production of a large amount of antibodies to the homologous booster antigen, whereas a little response to the heterologous antigen was noted. By this method high-titered anti-S serums, almost devoid of anti-V, or vice versa, were obtained.
3. The starch zone electrophoresis revealed the presence of V and S antibodies in the gammaglobulin fraction. V and S antibodies showed a slight difference in electrophoretic mobility, which made the separation of V and S antibodies possible.
