インフルエンザウイルスのマウス感染症

第8報 PR8株を経鼻噴霧感染した場合と経腹腔感染した場合とに於ける肺病変の差異の病理組織学的追求

抄録

Histopathological studies were made on mouse lung infected with the PR 8 virus intraperitoneally in an effort to differentiate the mode of infection from that of natural or intranasal infection.
First of all, the fact was noticed that the necrotic change or desquamation of the bronchial epithel, usually described as a characteristic feature of influenzal infections were not obvious when the virus was given intraperitoneally. However, the virus titers found with both lung tissue and bronchial washings were quite high and just comparable to the value obtained at the time of intranasal infection.
In order to explain the inconformities between the virus growth and development of consolidations described above, detailed study was carried out. Firstly, growth of virus, development of lung lesions and their correlations were carefully pursued against time. Secondaly, methylgreen and pyronine staining was used to examine lung tissues in addition to hematoxylin-eosin staining. As a result, it was found that the virus growth at the time of intraperitoneal infection was immediately accompanied by increased pyronine staining of bronchial epithel cell. This was concordant to the detected virus growth. In spite of this result, the necrosis or desquamation of these epithelial cell did not follow. Rather the development of consolidation, i.e. increase of histiocytes in alveorar septa was an event to be considered as a primary response.
The observation here made will point out the neccessity of plausible explanation for the desquamation of bronchial epithel which is usually found at the time of intranasal infections, but it was left for further studies. As a whole, the development of consolidation was understood as an immediate inflammatory response to the process of viral synthesis in bronchial epithel.