Abstract
Various purification procedures, such as ammonium sulfate precipitation, differential centrifugation, DEAE cellulose column chromatography and sucrose density gradient centrifugation, were applied in the purification of measles virus hemagglutinin. By combination of these various purification procedures, a highly purified measles virus hemagglutinin preparation was obtained.
The purified hemagglutinin showed antigenicity for hemagglutination inhibiting and neutralizing antibodies. This suggests that the protein coat of virus particles, split off by ether-tween 80 treatment, may have antigenicity both for hemagglutination inhibiting and neutralizing antibodies.
