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Volume 14, Issue 2
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[in Japanese]1964 Volume 14 Issue 2 Pages 55-59
Published: April 30, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (747K) -
[in Japanese]1964 Volume 14 Issue 2 Pages 61-67
Published: April 30, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSDownload PDF (1099K) -
(II) TIME OF APPEARANCE OF NEWLY FORMED VIRAL RNA AND PROTEIN IN THE LEAVES INFECTED BY CUCUMBER MOSAIC VIRUSTadao MISAWA, Sakari KATO1964 Volume 14 Issue 2 Pages 69-74
Published: April 30, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSThis report describes the process of formation of virus-RNA and virus-protein, and the changes of cyctoplasmic protein and nucleic acid metabolism in the tobacco leaves infected by cucumber mosaic virus.
(1) In the CMV-infected leaves, 15 hours after inoculation, a considerable amount of free infectious RNA is synthesized at first and about 30 minutes later virus particles are formed. Namely, multiplication of the infectious RNA seems to precede the formation of viral-protein after infection.
(2) The amount of free infectious RNA reached the maximum at about 18 hours after inoculation and decreased afterward as a consequence of combining with the protein. But the free infectious RNA was able to be detected slightly after 120 hours.
(3) Soluble cytoplasmic proteins of the inoculated leaves were isolated by paper electrophoresis. Eight fractions were separated from the leaves 72 hours after inoculation, but seven fractions from the healthy leaves. This new component moved faster than others, and was infectious.
(4) Nucleic acids of infected leaves were fractionated by ECTEOLA-cellulose column. Elution was carried out with 0.1M neutral phosphate buffer and NaCl solution in the range of 0.1-1.0M. Each fraction was measured for RNA concentration by the optical density at 200mμ. A new fraction which was not found in the control preparation was isolated from the inoculated leaves 2 days after inoculation. This fraction was eluted with 0.4M NaCl solution and not contained the viral-RNA. It is supposed that CMV-RNA was not eluted as a single fraction by this method.View full abstractDownload PDF (1177K) -
Tatsuo KITAWAKI1964 Volume 14 Issue 2 Pages 75-83
Published: April 30, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSVarious purification procedures, such as ammonium sulfate precipitation, differential centrifugation, DEAE cellulose column chromatography and sucrose density gradient centrifugation, were applied in the purification of measles virus hemagglutinin. By combination of these various purification procedures, a highly purified measles virus hemagglutinin preparation was obtained.
The purified hemagglutinin showed antigenicity for hemagglutination inhibiting and neutralizing antibodies. This suggests that the protein coat of virus particles, split off by ether-tween 80 treatment, may have antigenicity both for hemagglutination inhibiting and neutralizing antibodies.View full abstractDownload PDF (1011K) -
Jutaro TAWARA1964 Volume 14 Issue 2 Pages 85-88
Published: April 30, 1964
Released on J-STAGE: March 16, 2010
JOURNAL FREE ACCESSIn electron microscopic observation of serial ultra thin sections of intranuclear inclusion bodies of measles virus in dog kidney cells, filamentous fibers previously described in inclusion bodies were found to be of tubular structure, about 10-15mμ in inner and 15-20mμ in outer diameter. These fibers occur randomly at early stages of infection but gradually aggregate to a compact form, presenting crystalline arrangement in the serial cross sections.View full abstractDownload PDF (2928K)
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