Uirusu
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
RAPID ISOLATION AND IDENTIFICATION OF RS VIRUS BY MEANS OF MIXED TISSUE CULTURE AND IMMUNOFLUORESCENCE
Shiro ShigetaYoshio NumazakiNakao Ishida
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JOURNAL FREE ACCESS

1966 Volume 16 Issue 3 Pages 97-100

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Abstract
A simple and sensitive isolation procedure for respiratory viruses and a rapid identification of these isolates were intended.
RS virus was isolated more easily and rapidly by using mixed culture of HEp-2 and VERO cells rather than primary monkey kidney or human diploid cell cultures.
By means of immunofluorescent staining, identification of RS virus was possible within 24 to 48hours postinoculation. Whereas, apparent CPE was not detectable until 6 to 9 days.
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© The Japanese Society for Virology
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