1968 Volume 18 Issue 3 Pages 237-247
Mixed infection experiments of vaccinia subgroup viruses were done using plaque types and characters of A type inclusion as genetic markers. It revealed that A type inclusion marker could be divided into two parts; formation of inclusion matrix (In+ and In-) and virus attaching factor (V+ and V-). Recombinant between V and In markers was not found in over 200 progeny clones of mixed infection, but recombinants between plaque types and A type inclusion types were detected. Vaccinia IHD, which does not form A type inclusion by single infection, can rescue all V- type inclusion in mixedly infected cells and it results in formation of V+ type inclusion.