QUALITATIVE AND QUANTITATIVE ANALYSIS OF SMEAR PREPARATION ON COVER SLIP IN IMMUNOFLUORESCENT PROCEDURE

Abstract

Propriety of immunofluorescent procedures for the cells on a cover slip obtained from the VSV infected L cells in suspension was examined in qualitative and quantitative manner.
The cell number could be quantitatively recovered from the whole cover slip when transfered from the mother culture. The fixation, staining and washing procedures as used routinely in immunofluorescent study had no influence on cell number of the cover slip. Although the distribution of cell number varied from one microscopic field to another, there was no difference in proportion of the infected to the uninfected cells by means of the immunofluorescent staining.
These observations may give a warrant for the usual cover slip method, where only a portion of the cells in the mother culture is to be examined, that the cell population on the whole cover slip can be a representative of the mother culture.