Abstract
Shift of Japanese encephalitis antibody from IgM to IgG in the sera of the experimentally twice inoculated chicks with Japanese encephalitis (JE) virus was examined by the gel filtration of Sephadex G-200 column and following results were obtained.
1. Titer of HI antibody rised on 7 days after the first inoculation of JE virus and that increased rapidly after second inoculation and peak of the titer maintained for 2 months then decreased. Viremia was detected till 6 hours after first inoculation.
2. Hen was twice given JE virus and IgM antibody appeared 7 days after first inoculation, kept on rising, reached the peak 35 days after the first inoculation, that is 7 days after the second inoculation, then decreased, and disappeared on 120 days. IgG antibody appeared about two weeks after the appearance of IgM antibody and the titer became higher than that of IgM antibody 35 days after the first inoculation, then decreased gradually, and showed 1:16 of titer on 150 days.
3. We could obtain the chicks by fertilization from experimentally infected hen, having IgM and IgG of HI antibody of Japanese encephalitis. And the localization of antibody in the sera of chicks was determined by Sephadex G-200 gel filtration. IgM antibody was detected in chick serum, though IgG antibody was not detected.
