Abstract
1) A miniculture system has been devised using a continuous Vero cell line in the Leibovitz-15 (L-15) medium supplemented with bovine albumin fraction V. A highly humid atmosphere required for the incubation was obtained in a desicator by placing a small vial filled with water.
2) The above described system was applied to the titration of human sera for the poliovirus neutralization. A good correlation was obtained between this and the conventional tube method. Further more poliovirus serum neutralization by our system had a tendency of showing a little higher titer in the lower serum dilution area, and a little lower titer in the higher dilution area in comparison to the conventional tube method.
