実験的ヘルペスウイルス感染症に対するインターフェロンの効果

抄録

Herpes simplex virus (HSV) is reported to be relatively insensitive to the antiviral activity of interferon (IF), but no detailed information is available for it.
To obtain basic data on the clinical application of IF to human beings, studies were made on the effect of IF against the newly isolated HSV, HSV-TM strain, both in the tissue culture system of mouse embryo cells and in the mouse system with animals suffering from skin eruption and encephalitis.
1) Fifty per cent inhibition of the cytopathic effect, or virus yield, was observed when mouse embryo cells were treated with 10 units of IF and challenged with 50 TCID₅₀ of HSV-TM strain. A complete inhibition was seen when mouse embryo cells were treated with 100 units of IF and challenged with 5, 000 TCID₅₀ of HSV-TM.
2) Hairless mice of the 88-k strain were treated intraperitoneally with IF 24 hours before viral inoculation and daily thereafter. Daily inoculation with concentrated mouse IF, the maximum titer of which was 1.7×10⁵IU/0.3ml, did not inhibit the development of skin eruption in mice inoculated subcutaneously with 0.2ml of HSV-TM (10^5.5TCID₅₀/ml or encephalitis in mice inoculated intracerebrally with 0.03ml of HSV-TM (30 TCID₅₀).
These results imply that the administration of larger doses of interferon may be required for prophylaxis or treatment of HSV infection in animals.