ヒトサイトメガロウイルスに対するツニカマイシンの増殖抑制効果について
1985 年 35 巻 1 号 p. 53-61
詳細
1985 年 35 巻 1 号 p. 53-61
Replication of human cytomegalovirus (HCMV) was inhibited by 0.2μg/ml of tunicamycin (TM), which blocks the synthesis of glycoproteins. In the presence of TM, HCMV infected cells failed to permit viral DNA replication, expression of viral late antigens and formation of viral particles. Nevertheless, early cell rounding and the development of early immuno-fluorescent antigens were expressed in the presence of TM. Removal of TM resulted in restoration of virus titer and DNA synthesis within 72h. SDS-PAGE analysis revealed that TM-treated cells synthesized most early proteins at levels comparable to levels of synthesis in untreated cells.
The results suggest that TM blocks mainly late stage of the replicative cycle, and it may related with failing to synthesis a glycoprotein (s) appeared in the early phase of infection.
