Abstract
1) Using the photometric method, the incorporation of S antigen into incomplete virus and complete virus were compared with each other. At the same time, the amounts of S antigen formed in the infected tissues were also compared.
2) Decreasing the EID50/HA ratios of virus suspensions, the amounts of S antigen that can be released by ether treatment from virus particles decreased, and the correlation between EID50/HA ratio with CFS/CFV ratio of virus particles revealed that for each ten fold decrease in the former the CFS/CFV ratio decreases about two fold or so. The CFS/CFV ratios of each virus preparations were as follows:
SP virus: 1/1.5, UP1 vircus: 1/2.5, UP2 virus: 1/4.0, UP3 virus: 1/8.0, UP4 virus: 1/6.0, UP5 virus: 1/6.0.
3) The infected allantoic membranes contained large quantities of free S antigen, and the free-CFS/CFV ratios in the infected allantoic membranes were obtained as follows: SP: 6.0, UP1: 6.0, UP2: 4.0, UP3: 1.0, UP4: 2.0, UP5: 4.0.
4) The implications of these findings have been discussed.
