Abstract
In this communication a report is on the cellular receptor for mouse encephalomyelitis virus (GDVII strain).
The results are summarized as follows.
(1) The CDVII viruses absorbed on lecithin particles were eluted in normal saline solution by heating at 37°C for 15minutes.
(2) The purified lecithin showed a high inhibiting titer enough to inhibit 1 hemagglutination unit of GDVII virus by 0.2γ/cc.
The inhibiting action of lecithin was not found on the hemagglutination of influenza virus (PR8), mouse encephalomyelitis virus (FA) and Japanese B encephalitis virus (Nakayama strain), but to some extent on the hemagglutination of lymphogranulomatosis inguinalis virus when human group 0 erythrocytes and calcium chloride solution were used.
(3) The purified lecithin was made from human erythrocytes, mouse brain and soy bean. The inhibiting action of the lecithin showed an almost similar activity, irrespective of materials.
(4) The lecithin inactivated only hemagglutination of the virus with no regard to the infectivity, and the virus reserved its infectivity even in the state of combining with the lecithin.
(5) The lecithin was made up of glycerin, unsaturated fatty acid, saturated fatty acid, phosphoric acid and choline. The inhibiting action of lecithin was inactivated by phospholipase B, C and D but therefore, not inactivated by phospholipase A. And the inhibiting action was observed in a constitution of glycerin, saturated fatty acid, phosphoric acid and choline, but unsaturated fatty acid seemed to be unessential component.
(6) The receptor of erythrocytes was inactivated by phospholipase A, B, C and D.
(7) The lecithin was separated from erythrocytes and mouse brain, and CDVII virus infected mouse brain contained the lecithin 16 times less than normal mouse brains.
All these findings would indicate that lecithin are surmised to be concerned in the cellular receptor for mouse encephalomyelitis virus (GDVII strain).
