Abstract
In pursuing the growth of influenza virus in animals, particularly by means of hemagglutinin, or in performing the tissue culture experiment with influenza virus by means of infectivities again in tissue culture or of hemagglutinins, it was thought expedient to attempt to characterize the nature of both hemagglutinin inhibitors and growth inhibitory substances, detectable in animal sera or organs. The way how to destroy these inhibitors was also the matter of importance in performing these kinds of works. The study here described, summarized the results obtained along these lines of experiments.
In the first series of experiments, non-specific hemagglutination inhitors found in biological specimens have been divided into four groups. The first one was the so-called β-inhibitor and was contained in bovine serum. The representative of the fourth group was the guinea pig serum and its inhibitory action was destroyed with KIO4 but not with purified RDE. The second and the third groups were the already known α-inhibitors, but were differentiated each other by their specific inhibitory spectrum.
In the second set of experiments, the specimens tested above were examined for their growth inhibitory activity on the PR8 and CAM virus in Maitland's type tissue cultures. Chicken chorioallantoic membrne was used as the host tissue. Except the horse serum, all sera tested contained this kind of activity against these 2 viruses. Though the elucidation of this activity was not accomplished here, further development of this kind of study seems to be promissing.
Finally, the third approach involved here, was to apply the results obtained above in analyzing the distribution of these inhibitors against the PR8 virus particularly in mice. Lung, kidney and spleen contained fairly large amount of hemagglutinin inhibitors, which were susceptible to the action of RDE. Growth inhibitory substances were found only in serum and liver of mice and the latter was studied somewhat in detail with regard to its site of action. The inhibitory action, however, was not so high and the participation of this inhibitor in experimental infections were not elucidated so far.
