Abstract
According to a number of investigations published up to date, fresh active NDV had to be inoculated in a considerable amount to cause toxic effect or to produce a lung consolidation, and the inactivated virus was considered to be completely ineffective. In the present study, it was found that mice inoculated intranasally died of lung consolidation even when NDV had been inactivated previously by sodium ethylmercurithiosalicylate. Basing on this and other results obtained, a conclusion was drawn that infectivity, hemagglutination and activity to produce lung consolidation are respectively independent attributes of NDV, in which that concerned with lung consolidation was named “consolidation factor”.
Intravenous injection of fresh NDV caused a marked intestinal hemorrhage of mice, which was not produced with inactivated virus. Lung consolidation and intestinal hemorrhage were strikingly protected by an appropriate use of antonomic blocking agents such as tetraethylammonium bromide or chlorpromazine. From these facts it has been postulated that the pathological changes produced by NDV are results of an irritation in vegetative nervous system which may be caused by the components of NDV. It seems to be that in lung inoculated with NDV the consolidation factor may affect the vegetative nerve endings of respiratory tissues and its excess excitation results the consolidated lesions.
