Abstract
Within 1 h. after inoculation of HVJ Z strain of 8, 000-80, 000 HA u. into abdominal cavity of mice implanted with Ehrlich's tumor cells, many giant polynuclear cells appeared. The process of giant polynuclear tumor cell formation was a fusion phenomenon of agglutinated cells by HVJ.
The appearance of fused cells is in need of the following condition.
1) The tumor cells are vitable.
2) The hemolytic activity of HVJ is strong enough to destroy the tumor cell membranes severely.
3) When the concentration and hemolytic activities of HVJ are sufficient, the cells grow into fused cells at high concentration and degenerate into lysis at low concentration.
4) At the optimum concentration of virus and cells, shaking incubation is very effective for fusion, on the contrary under stational condition the cells degenerate into lysis.
