Abstract
1) Coxsackie virus (Group A, Type 2, Shida strain) can be serially cultivated over prolonged periods on mouse muscle tissue grafted to chick embryo chorioallantois.
2) After 11 or 12 passage by this method, the Coxsackie virus can be cultivated for more than 15 generations in chick embryo alone without reduction in virus titer.
3) The incubation period is longer with the chick embryo adapted strain when inoculated in the newborn mouse compared to the original strain.
4) The adapted strain does not show a cytopathogenic effect on L-cells but with in the range of 4 passages conducted in the study, a trend for growth of the L-cells was noted.
5) It was verified by neutralization tests using immune mouse serum that the adapted strain was Coxsackie virus Group A. Type 2.
