酸―アルカリ連続抽出法による海底堆積物中の有機態リン化合物の分画

抄録

Extraction patterns of six organic phosphorus (org.-P) compounds added to a marine sediment were studied using acid-alkali sequential extraction technique. The extraction patterns could be classified into three types : Acid extractable compounds were ATP and AEP (2-aminoethylphosphonic acid), cold alkali extractable ones were IHP (myo-inositol hexaphosphate), DNA and RNA, and hot alkali extractable one was PC (phosphatidylcholine). A portion of IHP, DNA and RNA, however, was not extracted completely because of the bound with and/or adsorption on co-existing organic matter. Approximately 40% of added ATP was hydrolyzed during the extraction procedures, while other org.-P compounds studied were stable and their hydrolysis ratios were less than 2%.
It was found from this study that the extraction patterns of org.-P compounds in marine sediments are strongly affected with the co-existing organic matter and/or with the extraction conditions, and that the acide-alkali sequential extraction technique gives a lower value than the present org.-P in sediments.