抄録
For comprehensive hazard assessment of environmental water samples with lower toxicity, simple bioassay using three human cell lines in combination with brief vacuum condensation have been developed. Based on the toxicity recovery test using 2 model compounds, it was shown that loss of the toxicity during the condensation process was negligible.
Then this bioassay system has been applied to environmental water samples from Lake Kasumigaura, Ayase River and Shinano River. With the condensation process, the cytotoxicity of the samples can be detected much more sensitively. The samples from Lake Kasumigaura showed the highest cytotoxicity in August and showed spatial variations depending on sampling points. In case of river water samples, higher cytotoxicity was associated with higher osmolarity. No significant correlation between the cytotoxicity and water quality data except osmolarity was observed.
These results indicate that this bioassay system is quite useful for toxicity assessment of environmental water with lower toxicity. However, to integrate this kind of indices in environmental management process, systematic and continuous accumulation of bioassay data on water samples with authentic water quality data must be essential.
