2002 年 25 巻 7 号 p. 409-415
The influence of algae on hydrogen production by a purple non-sulfur bacterium, Rhodobacter sphaeroides RV, the control of algal growth using a blue cellophane film and the hydrogen production by photosynthetic consortia were investigated by the batch culture using artificial substrate at 30°C. The influence of algae on hydrogen production was studied using pure culture of R. sphaeroides RV and coculture of algae and R. sphaeroides RV. Hydrogen production with the without a blue cellophane film was evaluated. Hydrogen production without the blue cellophane film in the coculture decreased by 15% compared with that in the pure culture. On the other hand, there was no significant difference in the amount of hydrogen production in cultures with the blue cellophane film. By absorbing the lights with wavelengths ranging from 600nm to 700nm, the blue cellophane film effectively controlled algal growth. The chlorophyll-a concentration in the coculture without the blue cellophane film increased by 1.5 times that of the initial concentration. On the contrary, the chlorophyll-a concentration in the co-culture with the blue cellophane film did not increase from the initial stage of the experiment. Moreover, hydrogen production was not inhibited in the pure cultures with or without the blue cellophane film. It is possible for the photosynthetic consortia to produce hydrogen from nonsterilized wastewater, although there is a decrease in hydrogen production of about 12% compared with that in the pure culture.