2001 年 37 巻 4 号 p. 135-140
Two sets of experiments were designed to study the digestion mechanism of methanogens acclimated by two kinds of carbon source, acetic acid and propionic acid. It was found that the utilization of propionic acid was a complex process, first changing into acetic acid prior to uptake by microorganism. In addition, possible inhibition of high concentration of the substrate was also demonstrated in this paper by using kinetic models. The inhibition behavior on the methane fermentation acts when the total volatile fatty acid (VFA) concentration is up to 3.6g/l, and the inhibition was completely observed for VFA 8.8g/l. The procedure described may be applied in defining theinhibition behavior of methane formation.